Figure 1

Figure 1: Selection of a pancreatic cancer cell line that express the sLe^a antigen to a high level. Expression of sLe^a antigen in a pancreatic cancer cell line together with PMF analysis. A, B) Western blotting analysis using the culture supernatant of 8 pancreatic cancer cell lines and expression of sLe^a antigen in the cell lysates. C) Capan-2 culture supernatant enriched by 1116NS19-9 antibody was subjected to SDS-PAGE and the resulting bands were visualized by silver staining. Relative band intensities of “1 μg” lane were analyzed and are depicted in the form of a histogram. The gel was cut into six pieces as highlighted by rectangles. Proteins extracted from each slice were analyzed by PMF. Black arrows show the peak of band intensities.