Figure 2: Enhanced uptake of AD12-FAM in cultured human cells (melanoma) subjected to heat shock. Human melanoma cells were grown at 37° C (dark gray) or heat shocked at 43° C for 1 hr in a tissue culture incubator (light gray) and allowed to recover for 5 hrs. AD12-FAM was added to the culture media and allowed to incubate for 1 hr at 37° C, followed by cell harvest and fluorescence measurements. Relative fluorescence values were plotted for the vehicle (DMSO) and AD12-FAM at three final concentrations: 1, 10, and 20 μM. Error bars indicate standard error of the mean for 6 runs.