Figure 3: In vivo imaging of local hyperthermia treatment in nude mice. Nude mice were locally treated with mild hyperthermia to the right posterior limb and following a recovery period of 5 hours, 6-carboxyfluorescein (FAM) (A, B) or AD12-FAM (C, D) were intravenously administered and imaged at 2 hrs using a fluorescence camera with appropriate excitation/emission filters. Immediately after this imaging, mice were euthanized and the skin removed to expose the underlying muscle (B and D). Arrow indicates the treated limbs. Regions of interest were drawn around the heat-treated limb for each of the 6-carboxyflurescein and AD12-FAM mouse subjects and fluorescence signal quantified at 30 minutes and 2 hrs post injection (p.i.) of live, intact, mice (E).