Insert Sequence Bias

Within the naïve library

• Partially due to the construction of the libraries [11,12]
• Partially due to the propagation of the libraries [13-15]

Selection Protocol Bias

Must take into account the final intended use of the selected targeting motif

• Appropriate negative selections [13]
• Stringency vs. yield[13]
• In Vitro - capture method/moiety [16]
• In Vivo - intra vs. extra-vascular [5]

Elution Protocol Bias

Elution method utilized

• Hydrophobic vs. hydrophilic elution [5,17,18]
• Competitive vs. non-competitive elution [19,20]
• Elution vs. enzymatic cleavage [21,22]