Figure 2: CTL mediated lysis of MCF-7 cells during cytotoxicity assay. Target MCF-7 cells were seeded at 5 × 104 cells per well in 48-well tissue culture plates, and then effector CTL were added at CTL:MCF-7 ratio 4:1. On following (a-d) or 3rd (a’-d’) day, target cells were carefully washed with HBSS and photographed on an inverted phase contrast microscope (scale bar: 50 µm). (a, a’) MCF-7 cells grown alone. (b, b’) MCF-7 cells incubated with CTL that had been stimulated with unloaded DC. (c, c’) MCF-7 cells incubated with CTL that had been stimulated with lysate-loaded DC. (d, d’) MCF-7 cells incubated with CTL that had been stimulated with trypsin digest-loaded DC.