Gene Amplicon Nucleotide positions in genomic DNA Primer sequence* Annealing Temperature (°C) Product size(bp) p53 Exon5 13005-13024 13295-13314 1)TGTTCACTTG TGCCCTGACT 2)AGCAATCAGTGAGGAATCAG 55 310 p53 Exon6 13271-13280                         13475-13494 1)TGGTTGCCCAGGGTCCCCAG 2)TGGAGGGCCACTGACAACCA 62 224 p53 Exon7 13941-13960                              14158-14177 1)CTTGCCACAG GTCTCCCCAA 2)AGGGGTCAGCGGCAAGCAGA 62 237 p53 Exon8 14442-14461 14579-14598 1)TCCTGAGTAGTGGTAATCTA 2)GCTTGCTTACCTCGCTTAGT 58 156 *1) Sense primer 2) Antisense primer

Table 1: Primers used for screening different exons of TP53.