Gene Symbol

Control

Cr (VI) treated

Fold change

HB2M

21.315 ± 0.117

21.842 ± 0.224

-

IL8

36.665 ± 0.321

29.026 ± 0.130*

+292.684

HMOX1

27.397 ± 0.136

25.4 ± .093*

+5.862

GDF15

26.673 ± 0.212

21.153 ± 0.215*

+67.378

FOS

33.544 ± 0.229

28.757 ± 0.103*

+40.544

RGS4

34.232 ± 0.202

30.691 ± 0.206*

+17.087

AXUD1

31.087 ± 0.084

28.287 ± 0.075*

+11.078

H3F3B

30.331 ± 0.189

28.063 ± 0.085*

+7.073

ANGPTL4

35.015 ± 0.241

32.2 ± 0.310*

+10.335

TNFRSF10D

27.235 ± 0.131

24.544 ± 0.234*

+9.485

GADD45A

26.291 ± 0.105

24.092 ± 0.096*

+6.746

GAS

26.127 ± 0.131

33.384 ± 0.124*

-104.17

DDIT4

28.138 ± 0.172

30.887 ± 0.247*

-4.576

KIAA1199

24.767 ± 0.239

29.408 ± 0.355*

-16.995

CXCL12

25.924 ± 0.141

30.841 ± 0.135*

-20.566

DKK1

22.619 ± 0.158

26.384 ± 0.132*

-9.259

HMGCR

30.413 ± 0.230

33.262 ± 0.114*

-4.905

KRT19

25.010 ± 0.129

29.197 ± 0.222*

-12.399

DHRS3

22.931 ± 0.318

26.164 ± 0.267*

-6.402

PTK2

27.950 ± 0.268

30.862 ± 0.406*

-5.128

TNS3

25.680 ± 0.169

30.639 ± 0.371*

-21.162

IRXL1

27.155 ± 0.198

31.224 ± 0.170*

-11.43

LMCD1

27.572 ± 0.201

31.893 ± 0.143*

-13.612

HOXA10

26.051 ± 0.220

28.967 ± 0.171*

-5.141

IFIT1

29.75 ± 0.425

35.132 ± 0.253*

-28.392

PPP1R3C

27.317 ± 0.298

29.058 ± 0.190*

-2.277

INSIG1

26.285 ± 0.293

30.156 ± 0.207*

-9.963

HMGCS1

26.772 ± 0.359

31.674 ± 0.167*

-20.353

SERPINB1

27.864 ± 0.135

31.006 ± 0.074*

-6.012

PRKD1

31.694 ±0.161

35.777 ± 0.230*

-11.317


Based on the microarray data, 29 genes were selected for real time PCR analysis to confirm their differential expression in the cells treated with Cr(VI). ‘+’ and ‘-’ represent overexpression and underexpression, respectively, of the genes in the Cr(VI) treated cells compared with the control cells. Data presented is the threshold cycle (CT) derived from real time PCR analysis and are mean + S.E. of four independent experiments. The fold change in expression of the individual genes in the Cr(VI) treated cells compared with the control was calculated as described in the text. Statistical analysis of the data was done by Student’s t test and a p value <0.05 was considered as statistically significant (*).
Table 1: Real Time PCR Confirmation of Differential Gene Expression in Human Dermal Fibroblasts Exposed to 5 µM Cr(VI).