Gene Symbol |
Control |
Cr (VI) treated |
Fold change |
HB2M |
21.315 ± 0.117 |
21.842 ± 0.224 |
- |
IL8 |
36.665 ± 0.321 |
29.026 ± 0.130* |
+292.684 |
HMOX1 |
27.397 ± 0.136 |
25.4 ± .093* |
+5.862 |
GDF15 |
26.673 ± 0.212 |
21.153 ± 0.215* |
+67.378 |
FOS |
33.544 ± 0.229 |
28.757 ± 0.103* |
+40.544 |
RGS4 |
34.232 ± 0.202 |
30.691 ± 0.206* |
+17.087 |
AXUD1 |
31.087 ± 0.084 |
28.287 ± 0.075* |
+11.078 |
H3F3B |
30.331 ± 0.189 |
28.063 ± 0.085* |
+7.073 |
ANGPTL4 |
35.015 ± 0.241 |
32.2 ± 0.310* |
+10.335 |
TNFRSF10D |
27.235 ± 0.131 |
24.544 ± 0.234* |
+9.485 |
GADD45A |
26.291 ± 0.105 |
24.092 ± 0.096* |
+6.746 |
GAS |
26.127 ± 0.131 |
33.384 ± 0.124* |
-104.17 |
DDIT4 |
28.138 ± 0.172 |
30.887 ± 0.247* |
-4.576 |
KIAA1199 |
24.767 ± 0.239 |
29.408 ± 0.355* |
-16.995 |
CXCL12 |
25.924 ± 0.141 |
30.841 ± 0.135* |
-20.566 |
DKK1 |
22.619 ± 0.158 |
26.384 ± 0.132* |
-9.259 |
HMGCR |
30.413 ± 0.230 |
33.262 ± 0.114* |
-4.905 |
KRT19 |
25.010 ± 0.129 |
29.197 ± 0.222* |
-12.399 |
DHRS3 |
22.931 ± 0.318 |
26.164 ± 0.267* |
-6.402 |
PTK2 |
27.950 ± 0.268 |
30.862 ± 0.406* |
-5.128 |
TNS3 |
25.680 ± 0.169 |
30.639 ± 0.371* |
-21.162 |
IRXL1 |
27.155 ± 0.198 |
31.224 ± 0.170* |
-11.43 |
LMCD1 |
27.572 ± 0.201 |
31.893 ± 0.143* |
-13.612 |
HOXA10 |
26.051 ± 0.220 |
28.967 ± 0.171* |
-5.141 |
IFIT1 |
29.75 ± 0.425 |
35.132 ± 0.253* |
-28.392 |
PPP1R3C |
27.317 ± 0.298 |
29.058 ± 0.190* |
-2.277 |
INSIG1 |
26.285 ± 0.293 |
30.156 ± 0.207* |
-9.963 |
HMGCS1 |
26.772 ± 0.359 |
31.674 ± 0.167* |
-20.353 |
SERPINB1 |
27.864 ± 0.135 |
31.006 ± 0.074* |
-6.012 |
PRKD1 |
31.694 ±0.161 |
35.777 ± 0.230* |
-11.317 |
Based on the microarray data, 29 genes were selected for real time PCR analysis
to confirm their differential expression in the cells treated with Cr(VI). ‘+’ and ‘-’
represent overexpression and underexpression, respectively, of the genes in
the Cr(VI) treated cells compared with the control cells. Data presented is the
threshold cycle (CT) derived from real time PCR analysis and are mean + S.E.
of four independent experiments. The fold change in expression of the individual
genes in the Cr(VI) treated cells compared with the control was calculated as
described in the text. Statistical analysis of the data was done by Student’s t test
and a p value <0.05 was considered as statistically significant (*). |