| P53 gene positions |
Primer Sequence |
Tm |
PCR Product size |
| Exon 4 (Codon 72) Arg>Pro27 |
Arginine allele
A1: 5'-TCCCCCTTGCCGTCCCAA-3'
A2: 5'-CTGGTGCAGGGGCCACGC-3' |
60°C |
141bp |
| Proline allele
P1:5'-GCCAGAGGCTGCTCCCCC-3'
P2:5'-CGTGCAAGTCACAGACTT-3' |
55°C |
177bp |
| Exon 5 (Codon168)
Cytosine deletion 28 |
F1(wt) 5'-CTACAAGCAGTCACAGCAC-3'
F2(mt)5'-CTACAAGCAGTCACAGCAA-3'
R (c) ATCAGTGAGGAATCAGAGG-3' |
55°C |
150bp |
| Exon 8(Codon 282) C>T (Arg>Trp) 28 |
F1(wt) 5'-CTTGTCCTGGGAGAGACC-3'
F2(mt) 5'-CTTGTCCTGGGAGAGACT-3'
R(c) 5'-GAGCTGGTGTTGTTGGGC-3' |
58°C |
205bp |
