Figure 3: Ureter hyperplasia in hormone treated mice. To determine the development of ureteral hyperplasia due to hormone treatment, 8 week old untreated mice of each genotype and hormone treated mice of corresponding genotypes were sacrificed and ureter taken and distended with fixative (10% formalin). (A) Ureteral hyperplasia grades reached. Hyperplasia of epithelial cells in the ureter before and after hormone treatment was graded using a similar grading scale to the bladder: grade 0: normal (2-3 cell layers); grade 1: slight hyperplasia (4 cell layers); grade 2: slight/moderate hyperplasia (5-8 cell layers); grade 4: moderate hyperplasia (9- 10 cell layers); grade 4: moderate/severe hyperplasia (11-12 cell layers); grade 5: severe hyperplasia (>12 cell layers). See text for details. (B) Example for average hyperplasia grade observed in each genotype. To determine the development of ureteral hyperplasia due to hormone treatment, 8-week-old untreated mice of each genotype and hormone treated mice (1 or 2 rounds) of corresponding genotypes were sacrificed and ureters were taken.