| |
|
T cells (No tumors)
(pg/ml) |
T+HEp2-vec
(pg/ml) |
T+HEp2-IκB(S32AS36A)
(pg/ml) |
| day |
T cells -/+IL2 |
|
- IFN-g |
+ IFN-g |
- IFN-g |
+ IFN-g |
| 15 |
- |
0.0±0.0 |
1.5±0.7 |
5 ± 2.8 |
11 ± 0.0 |
7.5 ± 4.9 |
| |
+ |
4 ±0.0 |
16.5±0.7 |
8 ± 1.4 |
185 ± 10 |
104.5±18 |
| |
|
|
|
|
|
|
| 19 |
- |
9 ±0.0 |
6 ± 4.2 |
3.5 ± 2.1 |
16 ± 0.0 |
5±0.0 |
| |
+ |
51.5 ±25 |
14.5 ± 9.2 |
10 ± 0.0 |
341.5 ± 20 |
231±42 |
| |
|
|
|
|
|
|
| 23 |
- |
0.0 ±0.0 |
0.0 ±0.0 |
0.0 ±0.0 |
23 ± 0.0 |
7.5±0.7 |
| |
+ |
9.5 ±0.7 |
13.5 ± 4.9 |
26.5 ± 2.1 |
1243 ± 25 |
465.5±16 |
Purified CD8+ T cells were treated with and without IL-2 (500 u/ml) overnight before their co-culture with and without IFN-γ (200 u/ml) treated vector alone and IκB(S32AS36A)
transfected HEp2 cells (E:T ratio 1:1). After two weekly stimulations with freshly supplemented IL2, the supernatants were removed from the CD8+ T /HEp2 cell cocultures
at the indicated days in the table and assayed for released GM-CSF by a specific and sensitive ELISA. IFN-γ treated HEp2 cell transfectants were washed three
times before they were added to IL-2 treated CD8+ T cells. No secretion of GM-CSF from vector-alone or IκB(S32AS36A) transfected HEp2 cells in the absence of CD8+ T cells
could be observed (data not shown). The p value for the difference between secretion of GM-CSF in the co-cultures of CD8+ T cells with control HEp2-vec and IκB(S32AS36A)
transfected HEp2 cells are less than 0.05. 1 of 3 representative experiments is shown in this table. |
