Figure 1: Schematic of culture set up and hypoxia exposure. NuFF and MDA231 cells were co-cultured in a 1:1 ratio in atmospheric oxygen (21% O2) or alternating 24 hour cycles of hypoxia (1% O2) and atmospheric oxygen (21% O2) for a period of 9 days. Subsequently, cells were lysed using a strong base and resulting ECM was investigated for changes in protein expression or was utilized as a substrate for HUVECs to form CLS.