Figure 6: Pro-angiogenic factors and MMPs were differentially expressed in CLS grown atop hypoxic and atmospheric ECM. (A) qRT-PCR for (i) VEGFA, VEGFR2, Ang1, Ang 2 and (ii) MMPs 1,2, and 9 and MT1-MMPin CLS grown on hypoxic and atmospheric de-cellularized ECM. (B) (i) Western blot for MT1-MMP and (ii) zymography for MMP1 and 2 in CLS grown on hypoxic and atmospheric ECM. Serum free media (SFM) was used as a control. *p≤0.05, **p≤0.01, ***p≤0.001.