| Transient expression |
Stable expression |
| Expression of transfected genes are usually within 16-96 h, but expression is not propagated through subsequent generations |
Heterologous DNA is integrated into the host genome and is stable throughout many generations |
|
Transfection requires a large amount of plasmid DNA |
Cell line development and testing requires an extensive period (4-12 months) |
| Expression level usually is relatively low
(5-100 mg/L) |
After amplification and high producer selection, expression level is relatively high (1,000-10,000 mg/L) |
|
Expression product is good for pre-clinical assessment, but no clear guidelines for clinical production |
Stable cells lines can be used for large scale manufacturing leading to clinical trials and commercialization |
| It takes only a short period to expression of a product for “Proof of Principal” demonstration |
|
