Figure 1: ZEBOV GP-specific Cytokine ELISPOT Response. ZEBOVGP-specific cytokine secreting splenocytes were detected in 5 different cytokine ELISPOT assays at 14 days after boosting the VSVΔG/ZEBOVGP immunized Balb/C mice. The splenocytes were stimulated with either peptide pool 1 (peptides 1-56), 2 (peptides 57-112), or 3 (peptides 113-167). Each pool contained 56 peptides, with each peptide at a concentration of 2 mg/ml. The bars represent the average number of ZEBOVGP-specific cytokine secreting splenocytes for 3 mice after subtracting the negative control well average. The assay was performed in triplicate, and the SEM is shown.