Figure 4: ZEBOVGP-specific Antibody Titres in Mice Vaccinated with Peptide # 98. Groups of Balb/C mice (n=5) were immunized with the various vaccines (PA= peptide alone, PK=peptide-KLH, PF=peptide + Freund’s, PKF=peptide-KLH + Freund’s) 3 times, 28 days apart. (A) Serum taken from mice on the first day of immunization (prebleed), and then on weeks 4, 8, and 11 were tested for the presence of ZEBOVGP-specific antibodies in an eVLP indirect ELISA. A 1:100 dilution of the serum was used for all samples. The negative control mice received only medium. The positive control mice received the rVSV (VSVΔG/ ZEBOVGP) vaccine, and were only immunized once, therefore only has a prebleed and 3 week serum sample. Each bar represents the average of the 5 mice in each group, with the error bars representing the standard deviation. The p values for the T test were determined by comparing the 4, 8, or 11 week sample to the prebleed within their own group, where *p≤0.05, **p≤0.005. (B) The prebleed, 4, 8, and 11 week ZEBOVGP-specific ELISA results are presented for each mouse (m1 to m5) in the PKF group. Mouse 1 (m1) is the animal that did not survive the MA-ZEBOV challenge.