Figure 5: ZEBOV GP-specific Cytokine ELISPOT Response in Mice Vaccinated with Peptide # 98. Groups of Balb/C mice (n=2) were immunized with the various vaccines (PA= peptide alone, PK=peptide-KLH, PF=peptide+Freund’s, PKF=peptide-KLH+Freund’s) 3 times, 28 days apart. At two weeks after the third injection, an IFN-g and IL-2 ELISPOT assay was used to determine the number of ZEBOVGP-specific cytokine secreting splenocytes in the various groups of immunized mice. The splenocytes were stimulated with 2 mg/ml of either peptide # 97, # 98, or # 99. The negative control mice received only medium. The positive control mice received the rVSV (VSVΔG/ZEBOVGP) vaccine, and were immunized once followed by a boost 28 days later, then 2 weeks later used in the assay. The bars represent the average number of ZEBOVGP-specific cytokine secreting splenocytes for the 2 mice in each group after subtracting the average of the negative control wells. The assay was performed in triplicate.