Figure 4: Functional expression of cytokines in mouse splenocytes from each indicated treatment groups after ex vivo stimulation with the immunodominant ZEBOV GP peptide. Lymphocytes were gated according to the expression of CD4 or CD8 (upper panel). CD4+ and CD8+ cells were then analysed for the expression of IFN-γ, TNF-α and IL-2 (lower panels). Numbers in the grid in the upper right quadrant represent the percent of cells in each quadrant.