Table 1

Antigen	Size (kDa)	Functional properties^a	Infectious form association^b	Reference
A4L	39	Viral core protein synthesized late post-infection and involved in viral core assembly.	IMV	[50]
A27L	14.0	Required for formation and viral assembly.	IMV	[51]
H3L	37.5	C-terminal transmembrane protein involved in morphogenesis.	IMV	[52,53 ]
F9L	24	Related to L1R. Essential for virus entry and cell fusion.	IMV	[54]
L1R	27.3	Myristoylated type I membrane protein involved in viral assembly.	IMV	[55]
A33R	20.5	Type II membrane protein involved in actin tail formation.	EEV	[56]
A56R	85	Related to hemagluttinin	EEV	[57]
B5R	35.1	Type I membrane protein involved in viral egress.	EEV	[58]

^aAll antigens described have been shown to be essential for viral replication. ^bThe intracellular mature virion (IMV) is retained within the infected cell, while the extracellular enveloped virion (EEV) form of the virus is actively secreted from cells contributing to the efficient dissemination of the virus in vitro [59] and in vivo [60]. Although not clearly understood, it has been suggested that the IMV form is responsible for host-to-host spread and the EEV form is thought to be primarily involved in long-range spread within the host [61].

Table 1: Biological properties of the cloned VACV genes.