Figure 8: Validation of microarray data by Real-time PCR expression profiles for selected genes. The bar graph shows the log1.5 transformed genomic fold changes (B. anthracis spores-exposed sample/un-exposed control) with ± SEM. The white and gray bars represent the average-fold changes measured by the microarray and realtime PCR platforms, respectively. Gene expression values in the real-time PCR experiment are normalized to mRNA levels of GAPDH, the housekeeping gene.