Biofunction: Cell death (p=3.5 E-4) GenBank Accession Gene symbol 2 hrs 4 hrs 6 hrs 8 hrs 24 hrs AA393203 ATF1 0.39 1.14 0.93 0.89 1.52 AA952981 ATP2B1 0.90 1.83 1.05 1.33 0.89 AB002376 ERC2 1.20 0.83 1.73 0.23 2.51 AI004969 ATF1 1.15 1.69 1.05 2.64 1.54 AI050734 ALDH1A1 3.69 0.57 1.99 1.16 1.86 AI829016 CD59 0.87 0.50 0.86 0.58 0.76 AW665276 CAST 0.87 1.54 1.72 1.55 1.47 BE350967 CENTG3 3.29 2.23 1.13 1.42 1.35 BF677029 BCL2A1 25.53 23.67 11.02 34.69 6.31 BG397697 ADRBK1 4.47 1.74 1.18 1.41 1.53 NM_001223 CASP1 3.16 4.13 2.40 7.81 1.13 NM_004034 ANXA7 2.35 0.90 1.60 1.72 0.66 NM_004360 CDH1 0.45 0.53 1.21 0.26 1.46 NM_006614 CHL1 6.13 1.97 1.33 3.39 3.48 NM_012099 CD3EAP 0.73 0.87 0.47 0.77 0.97 U75968 DDX11 1.46 0.75 0.78 0.47 4.19 Biofunction: Cell morphology (p=4.6 E-4) GenBank Accession Gene symbol 2 hrs 4 hrs 6 hrs 8 hrs 24 hrs AA569764 CDH1 0.73 1.03 1.03 0.70 1.17 AB022659 CEP170 2.37 1.48 1.80 1.23 1.99 BG483248 AQP4 2.60 1.07 1.39 0.24 1.03 NM_001748 CAPN2 4.03 2.39 1.99 0.87 0.99 Biofunction: Cell-to-cell signaling and interaction (p=3.5 E-3) GenBank Accession Gene symbol 2 hrs 4 hrs 6 hrs 8 hrs 24 hrs BE907932 CD63 2.77 1.27 0.44 0.62 1.30 BF677029 BCL2A1 25.53 23.67 11.02 34.69 6.31 NM_004360 CDH1 0.45 0.53 1.21 0.26 1.46 W46422 CD59 4.29 1.12 1.82 1.02 2.60 Biofunction: Cell growth and proliferation (p=7.6 E-6) GenBank Accession Gene symbol 2 hrs 4 hrs 6 hrs 8 hrs 24 hrs AF038193 ARL3 2.47 1.30 1.62 1.95 2.07 AU098377 ARL3 1.51 0.93 1.01 1.08 2.52 AW005824 ABTB1 1.94 1.89 1.42 3.94 1.01 NM_001124 ADM 2.27 0.42 2.29 3.54 1.44 NM_012099 CD3EAP 0.73 0.87 0.47 0.77 0.97 Biofunction: Molecular transport (p=2.4 E-2) GenBank Accession Gene symbol 2 hrs 4 hrs 6 hrs 8 hrs 24 hrs AB002376 ERC2 1.20 0.83 1.73 0.23 2.51 AW474551 CAMLG 1.27 1.56 1.47 0.78 1.84 W46422 CD59 4.29 1.12 1.82 1.02 2.60

Table 1: Functional classification of the genes significantly altered by exposure to B. anthracis spores. The five most significantly enriched biofunctions with corresponding GeneBank accession and Gene Symbol are recorded. The gene ontology enrichment p-values are recorded inside the parenthesis with each function. The fold change is: transcriptomic expression of treated/ control cells of donor i at time j; where i=donor 1, 2, 3 and 4; and j=2 hrs, 4 hrs, 6 hrs, 8 hrs and 24 hrs.