| BOTE Project Event1 |
Total Sample No.2 |
Samples |
True Blanks |
Negative Controls |
Positive Controls |
| RV-PCR and Culture Pos. |
RV-PCR and Culture Neg. |
RV-PCR Pos. and Culture Neg. |
RV-PCR Neg.
and Culture Pos.3 |
Total Agreement4 (%) |
False Pos.4 (%) |
False Neg.4 (%) |
RV-PCR and Culture Neg. |
RV-PCR and Culture Neg.5 |
RV-PCR and Culture Pos. |
| VHP |
44 |
30 |
11 |
1 |
2 |
41/44
(93.2%) |
1/44
(2.3%) |
2/44
(4.5%) |
5/5 |
3/3 |
3/3 |
| Bleach |
47 |
3 |
44 |
0 |
0 |
47/47
(100%) |
0/47
(0%) |
0/47
(0%) |
3/3 |
2/3 |
3/3 |
| CD |
68 |
0 |
68 |
0 |
0 |
68/68
(100%) |
0/68
(0%) |
0/68
(0%) |
5/5 |
4/5 |
5/5 |
| Total |
159 |
33 |
123 |
1 |
2 |
156/159
(98%) |
1/159
(0.6%) |
2/159
(1.4%) |
13/13 |
9/11 |
11/11 |
1VHP, vaporous hydrogen peroxide; Bleach, pH-adjusted bleach; CD, chlorine dioxide.
2Total sample number included surface and QC samples and did not include True Blanks or laboratory negative and positive controls.
3For two Post-VHP samples, the culture portion required PCR analysis of the concentrated enrichment culture to obtain positive results.
4Note that each sample was divided into two equal parts for parallel RV-PCR and culture analyses; as a result, variability could have been observed for the samples with
low spore levels. Percentages were based on surface and QC samples and did not include True Blanks or laboratory negative and positive controls.
5For two of eleven negative controls, culture results were positive but RV-PCR results were negative, suggesting cross-contamination occurred for the culture portion.
Abbreviations: Pos, Positive; Neg, Negative. |
