Figure 4: The diagrams (a, e, i, m) showing pG1-c-Kit-GFP-encoding transgene insert (a) and control-GFP inserts (e, i, m) (circled). Testes were directly observed under microscope using GFP filter on day 56 post GCT (b, f, j, n). Cross section of seminiferous tubules on day 56 post GCT showing expression of c-Kit fusion protein by the differentiated SSCs located at the periphery (c,g). However, the testicular cross-sections in control-GFP recipient testis (k, o) did not show the expression of GFP. C-Kit-GFP expressing SGCs isolated from c-Kit-ORF-GFP (d) c-Kit-GFP (h) and expressing testes on day 56 post GCT. Figures shown are the representative pictures from two different experiments (Scale bar: 50 μm) .