Figure 6: (A).Confirmation of GFP mRNA expression in SSCs of c-Kit-ORF-GFP (Lane: 1, 312 bp), 1.1 kb control-GFP introduced (Lanes: 2, 3), wild type (Lane: 4) mice testis and no RT enzyme control (Lane: 5). GFP-mRNA transcript was seen only in the SSCs of c-Kit-ORF-GFP insert introduced mice. GAPDH was used as internal loading control. None of the control insert introduced SSCs showed c-Kit expression (Lanes: 2-5). (B). Western blot analysis of c-Kit-GFP fusion protein expression in SSCs of c-Kit-ORF-GFP (Lane: 2, 172 kDa), 1.1 kb control-GFP insert introduced and wild type (Lanes: 3,4) mice testis (Lane: 5, 6). GFP protein expression was seen only in the SSCs of c-Kit-ORF-GFP insert introduced mice testis. In negative controls (Lanes: 7, 8) primary antibody was replaced with normal mouse serum. β-actin was used as internal loading control. Figure shown is the representative pictures from two different experiments.