A. IRK cells expressing PARP-1-N-term-EGFP were treated with 200 μM hydrogen peroxide for 5 minutes and examined live by CLSM. Panel 1, nuclei of untreated cell (control). Panel 2, Nuclei of cell displaying membrane blebbing indicated by arrows. Panels 3 and 4 cell with apoptotic fragment in cytoplasm. Scale bar represents 75 μM. B. Localisation of fusion protein following treatment with 200 μM hydrogen peroxide C. Non-transfected IRK cells treated with 200 μM hydrogen peroxide, fixed, permeablised and stained for poly (ADP-ribose) using 10H primary antibody and FITC conjugated secondary antibody. Scale bar represents for panels 1 and 2 is 6 μm and for panels 3 and 4 is 22 μM. Each panel is representative of atleast 60 cells from three separate experiments.
