Level

K’

T

(Rs)

A: pH of mobile phase

2.8

-0.2

2.5

1.51

2.37

3

0

2.6

1.53

3

3.2

0.2

2.2

1.58

2.35

S.D n=6

 

0.21

0.04

0.01

B: Flow rate (mLmin-1)

0.8

-0.2

2.1

1.56

2.32

1

0

2.6

1.53

3

1.2

0.2

2.5

1.51

2.37

S.D n=6

 

0.28

0.03

0.03

C: Percentage of water in mobile phase (V/V)

90/10

-20

2.7

1.52

2.39

70/30

0

2.6

1.53

3

80/20

-10

2.4

1.57

2.32

S.D n=6

 

0.21

0.03

0.04

D: Wavelength (nm)

220

-5

2.7

1.52

2.39

225

0

2.6

1.53

3

230

5

2.5

1.59

2.33

S.D n=6

 

0.14

0.04

0.03

K’= Capacity factors, T = Tailing factor, Rs = Resolution
Table 5: Robustness of the proposed method.