HPLC

 

TLC-UV Densitometry

Parameter

A

G

Reference values

Parameter

A

G

retention time tR

16.3 ± 0.1

10.3 ± 0.1

 

Rf

0.14

0.66

Resolution Rs

9.86

2.63

Rs≥2

 

Resolution Rs

 

7.7 (calculated between A and G) 2.27
(calculated between G and AD)

Tailing Factor

1.11

1.2

T≤2

 

Capacity Factor (K')

3.5

22.75

Asymmetry Factor

1.33

0.98

 

 

Selectivity α

 

6.50

Capacity Factor (K')

6.13

3.52

1<K'<10

 

Tailing Factor

1.25

1

Selectivity α

1.33

1.74

α>1

 

 

 

 

Column Efficiency N

2988.4

4261

N>2000

 

 

 

 

HETP (Height Equivalent to theoretical plate)

0.084

0.059

 

 

 

 

 

Table 1: System suitability parameters for Ambroxol HCl and Guaifenesin by the proposed HPLC and TLC-spectrodensitometric method.