Mix No

A µg.ml-1

PLS

PCR

AD µg.ml-1

PLS

PCR

G µg.ml-1

PLS

PCR

GD µg.ml-1

PLS

PCR

1

7.50

99.29

99.40

2.40

98.85

98.95

40.00

102.51

102.15

12.00

98.57

98.58

2

9.00

99.44

99.46

3.00

95.90

95.76

45.00

99.03

99.05

9.00

100.74

101.07

3

6.75

99.10

98.87

2.70

99.62

99.69

55.00

101.50

102.04

12.00

96.99

96.49

4

6.75

101.91

101.83

3.30

101.32

101.38

60.00

100.93

101.08

11.00

100.81

100.72

5

7.50

100.18

100.25

3.60

100.22

100.29

60.00

98.24

98.03

8.00

106.21

106.56

6

6.00

101.01

100.78

3.00

101.47

101.30

55.00

100.77

101.22

11.00

99.40

99.59

7

8.25

100.76

100.79

3.30

99.81

100.12

45.00

99.60

99.50

8.00

100.85

100.09

8

8.25

101.7

101.74

2.70

100.72

100.97

40.00

99.39

99.23

9.00

101.12

100.77

Mean

 

100.42

100.39

 

99.74

99.81

 

100.25

100.29

 

100.59

100.48

R.S.D.%

 

1.093

1.094

 

1.782

1.833

 

1.419

1.531

 

2.684

2.877

Table 2: Results of the analysis of the mixtures of the validation set of Ambroxol HCl, Ambroxol HCl degradate, Guaifenesin and Guaicol by the proposed PLS and PCR models.