Parameters

HPLC Method

TLC Spectrodensitometric Method

Ambroxol HCl

Guaifenesin

Ambroxol HCl

Guaifenesin

Precision

 

 

 

 

Repeatability

1.388

1.984

0.851

1.274

Intermediate precision

1.577

2.082

1.807

1.699

Calibration

 

 

 

 

Range

5-80 µg.ml-1

10-150  µg.ml-1

1-7 µg/band*

2-10  µg/band*

Slope (a)

0.0515

0.033

0.3220

0.2608

Intercept (b)

-0.0414

-0.012

0.3472

0.6443

Correlation Coefficient (r)

0.9999

0.9999

0.9999

0.9997

Accuracy

99.95 ± 0.821

100.07 ± 1.093

99.89 ±1.029

100.00 ± 1.161

Specificity

100.40 ± 0.964

100.00 ± 1.041

100.00 ± 1.179

99.90 ± 0.499

LOD 

0.757

1.630

0.060

0.110

LOQ

2.295

4.941

0.182

0.334

Regression equations for Ambroxol HCl and Guaifenesin by TLC-spectrodensitometry followed second order equation:
For Ambroxol HCl, P= -0.0179C2+0.4652C+0.1324
For Guaifenesin, P= -0.0172C2+0.4677C+0.1386
Table 5: Validation results of the proposed HPLC and TLC-spectrodensitometric methods method.