Proposed methods for Ambroxol HCl

Reported methods for A

Proposed methods for Guaiphenesin

Reported methods for G

HPLC

TLC

PLS

PCR

Reporteda

HPLC

TLC

PLS

PCR

Reportedb

Mean

99.95

99.89

100.42

100.39

100.22

100.07

100.00

100.25

100.29

100.28

RSD

0.821

1.029

1.093

1.094

0.754

1.093

1.161

1.419

1.531

1.066

Conc. Range

5-80 µg.-1

1-7 µg.-1

6-9 µg.-1

6-9 µg.-1

 

10-150 µg/band

2-10 µg/band

40-60 µg.-1

40-60 µg.-1

 

n

5

7

8

8

4

6

9

8

8

7

Variance

0.674

1.059

1.195

1.197

0.569

1.195

1.348

2.014

2.344

1.136

Student's t test

0.508 (2.365)*

0.556

(2.262)*

0.325 (2.230)*

0.325

 (2.23)*

 

0.350

(2.201)*

0.496 (2.145)*

0.046 (2.600)*

0.014 (2.600)*

 

F-test

1.185 (9.117)*

1.861

(8.941)*

2.100 (8.887)*

2.104 (8.887)*

 

1.052 (4.387)*

1.187 (4.147)*

1.773 (4.207)*

2.063 (4.207)*

 

n is the number of trials.
* These values represent the corresponding tabulated values of t and F at p=0.05.
a.Reported method was a spectrophotometric method for A by measuring the absorbance of A in 0.1M HCl at 244 nm.
b.HPLC for the determination of G on C18 column, mobile phase (KH2PO4-methanol, 55:45; v/v, pH3), Flow rate 1 ml/min UV detection at 220 nm.
Table 6: Statistical comparison of the results obtained by the proposed methods and the reference reported methods for pure samples of Ambroxol HCl and Guaifenesin.