ID no.

Concentration (µg/mL)

 

Regression Parameters

0.25

0.50

1.00

1.50

2.00

4.00

Intercept

Slope

r2

1

0.24

0.56

0.95

1.53

2.08

3.93

0.0154

0.0005

0.9971

2

0.27

0.52

1.02

1.50

2.02

3.92

0.0267

0.0006

0.9954

3

0.23

0.53

0.96

1.44

2.11

4.20

-0.0532

0.0010

0.9975

Mean

0.24

0.53

0.98

1.49

2.07

4.01

-0.0037

0.0007

0.9967

SD

0.02

0.02

0.06

0.07

0.13

0.15

0.0432

0.0003

0.0011

% RSD

8.96

4.29

6.30

4.68

6.07

3.76

 

 

 

% Bias

-3.20

6.40

-2.33

-0.78

3.33

0.29

 

 

 

Table 2: Summary of calibration curves for tramadol with back calculated concentrations.