Nominal concentration (µg/mL)

Found concentration
(µg/mL)

Precisiona
(RSD)

Accuracy
(%)

 

 

 

 

Intra-assay (n = 5)

 

 

 

LQC (0.75)

 

 

 

Day 1

0.74

6.06

99.20

Day 2

0.75

5.75

100.53

Day 3

0.75

9.79

100.27

 

 

 

 

MQC (1.50)

 

 

 

Day 1

1.53

4.30

101.67

Day 2

1.60

7.41

106.83

Day 3

1.48

1.40

98.50

 

 

 

 

HQC (3.00)

 

 

 

Day 1

3.14

3.11

104.53

Day 2

3.21

1.95

107.13

Day 3

3.00

7.25

99.92

 

 

 

 

Inter-assay (n = 15)

 

 

 

 LQC

0.75

0.77

100.44

MQC

1.53

3.98

102.22

HQC

3.12

3.43

103.89

aRSD: relative standard deviation
Table 3: Intra- and inter-assay precision and accuracy for tramadol in human saliva.