Parameters PRG MC
Range (ng/band) 1500-7500 150-750
Retention time (min) 6.4 7.9
Resolution 2.02
Detection limit (ng/band) 177 27
Quantitation limit (ng/band) 535 82
Accuracy (%) 99.86-100.24% 97.83-99.54%
  Precision (%RSD)
Intra-day (n=3) 0.15-0.28 0.82-1.81
Inter-day (n=3) 0.29-0.65 0.48-1.21
Instrument precision (%RSD) 0.51% 1.32%
Specificity Specific Specific
Table 3: Summary of validation parameters of developed HPTLC method.