Figure 1: Chemical formula of ligands and adsorption rates for albumin, IgG, IgM, and fibrinogen.
The chemical structure of ligands is shown. There were 4 thiol compounds (L–α–3–thienylglycine, β–2–thienyl–DL–serine, β–2–thienyl–D–alanine, and 2–thiophene ethylamine) and 5 types of amino acids (glycine, L-alanine, L-serine, L–phenylalanine, and L–tryptophan). The adsorbent (1.0 mL) was placed into a 2.5–mL column, and 3.0 mL of healthy human plasma was run through the column at a flow velocity of 0.3 mL/min for 10 min, and all of the plasma that was eluted from the column was collected. Protein adsorption rate (%) was calculated as (Cin‚ąíCout)/Cin×100, where Cin is the concentration of the solute before adsorption and Cout is the concentration of the solute after adsorption. Mean adsorption rates for samples from 3 healthy volunteers are shown. IgG and IgM adsorption relative to fibrinogen adsorption was evaluated using the mean adsorption rates for IgG, IgM, and fibrinogen.