dThdPase activitya)

Immunohistochemistryb)

Histopathological

 

Case

Tumor

Normal

Epithelial

Stromal

diagnosisc)

Organ

No.

tissue

tissue

cells

cells

 

Uterine cervix

1

7.0

7.5

-

+

EC Ad Ca, HD

 

2

172.5

NOSd)

-

++

EC Ad Ca, PUD

 

3

180.0

6.7

++

+

Sq Ca

 

4

236.0

11.0

±

++

Sq Ca

 

5

241.0

14.2

++

++

Sq Ca

 

6

243.0

7.1

++

-

Sq Ca

 

7

260.6

168.2

++

+

Sq Ca

 

8

341.5

1.8

++

+

Sq Ca

 

9

343.0

31.0

+

+

Sq Ca

 

10

344.5

9.4

+

+

Sq Ca

 

11

410.5

17.7

+

±

EC Ad Ca, PUD

 

12

572.3

26.6

++

++

Sq Ca

 

13

602.5

13.4

++

++

Sq Ca

Endometrium

1

15.1

43.9

-

+

EM Ad Ca

 

2

20.6

28.0

-

-

EM Ad Ca

 

3

41.5

6.2

-

+

EM Ad Ca

 

4

50.8

8.4

+

++

EM Ad Ca

 

5

56.8

7.5

±

±

EM Ad Ca

 

6

70.0

1.3

-

+

EM Ad Ca

 

7

81.6

11.1

-

+

EM Ad Ca

 

8

81.8

8.3

-

++

Serous Ca

 

9

111.2

73.2

+

++

EM Ad Ca

 

10

117.6

9.2

+

++

EM Ad Ca

 

11

120.0

NOS

+

+

EM Ad Ca

 

12

129.0

6.5

+

++

EM Ad Ca

 

13

135.0

5.1

-

++

EM Ad Ca

 

14

161.4

8.3

+

++

EM Ad Ca

 

15

187.0

24.4

+

++

EM Ad Ca

 

16

192.5

59.5

+

+

EM Ad Ca

 

17

200.0

10.6

++

++

EM Ad Ca

 

18

203.0

18.7

+

+

EM Ad Ca

 

19

229.0

28.6

+

++

Clear cell Ca

 

20

339.0

12.4

+

++

Serous Ca

Ovary

1

11.5

NOS

-

+

Mucinous Ca

 

2

12.3

NOS

-

+

Endomet Ca

 

3

21.3

NOS

+

+

Clear cell Ca

 

4

42.5

11.6

+

-

Mucinous Ca

 

5

48.0

NOS

-

++

Serous cyst Ca

 

6

61.7

0.0

+

++

Mixed epithel Ca

 

7

73.0

NOS

+

+

Clear cell Ca

 

8

75.0

NOS

++

+

Clear cell Ca

 

9

101.0

14.8

+

-

Clear cell Ca

 

10

143.0

NOS

NOS

NOS

Serous cyst Ca

 

11

238.4

NOS

-

++

Serous cyst Ca

 

12

12852.5

19.3

++

++

Serous cyst Ca


a) dThdPase activity is expressed as μg FU produced per mg protein per hour.
b) Immunohistochemistry is scored by the number of cells showing positive staining for anti-dThdPase mAb: (-), negative staining; (±), weak and less than 10% positive cells; (+), 10% to 50% positive cells; (++), more than 50% positive cells.
c) EC Ad Ca, endocervical adenocarcinoma; HD, highly differentiated; EMOD, endometrioid carcinoma; PUD, poorly differentiated adenocarcinoma; Sq Ca, squamous cell carcinoma; EM Ad Ca, endometrial adenocarcinoma; Serous Ca, serous adenocarcinoma; Serous cyst Ca, serous cyst adenocarcinoma; Endomet Ca, endometrial adenocarcinoma; Clear cell Ca, clear cell carcinoma; Mucinous Ca, mucinous cyst adenocarcinoma; Mixed epithel Ca; mixed epithelial adenocarcinoma.
d) NOS, no specimen
Table 1: Activity of a pyrimidine nucleoside phosphorylase, dThdPase, immunohistochemistry in gynecological tumors and adjacent normal tissue.