Samples                                                     Extraction by shaker
                                                    IC50 values (mg/ml)
                                       DPPH                           Metal Chelation
             BGS-1              BGS-2              BGS-1              BGS-2
BGS-H2O 144.10 cb ± 5.11 135.01 cb ± 5.01 371.62 ca ± 4.99 390.17 cb ± 4.91
BGS-EtOH
Aquous EtOH (80%)
157.42 bc ± 6.03 163.52 ab ± 5.99 143.59 bb ± 3.72
151.05 ab ± 5.11
442.18 ba ± 3.69
461.27 ab ± 5.03
421.03 ab ± 3.99
402.62 bb ± 4.15
                                                      Extraction by reflux
BGS-H2O 143.02 cb ± 5.42 134.17 cb ± 4.82 372.19 ca ± 4.29 390.01 cb ± 5.12
BGS-EtOH
Aquous EtOH (80%)
157.01 bc ± 5.82 160.28 aa ± 6.17 139.32 bc ± 4.17
152.87 ab ± 4.92
444.16 ba ± 4.10
455.17 ac ± 5.23
417.39 ac ± 3.62
400.18 bc ± 5.12
                                                      Extraction by ultrasound
BGS-H2O 50.01 ca ± 5.32 139.43 ca ± 5.28 370.16 ca ± 5.12 401.1 ca ± 5.20
BGS-EtOH
Aquous EtOH (80%)
166.14 bb ± 5.82 168.28 aa ± 5.41 150.14 ba ± 4.21
159.20 aa ± 4.99
443.18 ba ± 4.12 465.13 aa ± 5.32 442.18 aa ± 3.71
411.03 ba ± 4.21
Values (mean ± SD) are average of three samples of each BGS sample, analyzed individually in triplicate (n = 1x3 x 3), (P < 0.05); Superscript letters within the same row indicate significant (P< 0.05) differences of means within the extracting solvent; Subscript letters within the same column indicate significant (P< 0.05) differences of means among the extracting technique.
Table 6b: DPPH and Metal Chelation of bitter gourd seed.