Table 5

Organism	# clones sequenced (expired lot)^a	PCR (expired lot)^b	# clones sequenced (new lot)	PCR (new lot)
Acinetobacter baumannii CP001172	5	+	0	-
Bacillus cereus CP000227	3	+	2	+
Bacillus licheniformis EF685205	15	+	58	+
Enterococcus faecium GQ405286	94	+	0	-
Brevibacillus choshinensis AB038650	0	+	2	+
Bacillus subtilis	0	-	38	+

^a117 cpn60 isolates from the expired Product A and 100 cpn60 isolates from the new lot were sequenced. Bacterial sequences were determined by BLAST analysis using NCBI or against the cpn60 database http://www.cpndb.ca/cpnDB/home.php) (Hill et al, 2004)
^bPCR represents a positive signal against total extracted consortia DNA using cpn60 primers specific for that species

Table 5: Consortia composition by cpn60 isolate sequencing.