Variant

KD (M)

Kon (1/Ms)

Koff (1/s)

R2

rhBAFF

3.63E-08

9.64E+02

3.50E-05

0.872721

Trx

 ---

 ---

 ---

0*  

M1

3.18E-07

2.93E+02

9.34E-05

0.951368

M2

1.17E-06

4.18E+02

4.88E-04

0.914619

M3

5.33E-07

4.60E+02

2.45E-04

0.959939

M4

 ---

 ---

 ---

0*  

Table 1: The binding kinetics of the BAFF receptor TACI to BAFF and BAFF mutant using the ForteBio system Off-rate kinetics (koff) were measured by saturating the chip with TACI and then monitoring dissociation after switching to buffer. On-rate kinetics (kon) was measured using 3 kinds of dilutions of BAFF or BAFF mutant. KD, the equilibrium dissociation constant, was calculated as Koff/ Kon. Trx、rhBAFF 、M1、M2、M3 and M4: see Figure 2.
The bar (---) means the data is too limited to be analyzed.
*Curve equations could not be established to fit with the experiment data and fitting accuracy (R2) showed only zero.