T cells isolated from murine splenocytes using the T Cell isolation kit were co-stimulated with PMA (5nM) and ionomycin (1 μg/ml). After 24 hrs, siRNA targeted at cyclophilin B was transfected into the activated primary T cells using the HVJ-E. Real-time PCR evaluation was performed at 48 hrs after the transfection
Figure 4: Delivery of siRNA into activated T cells using HVJ-E vectors.