Three kinds of siRNA sequences (1-3) selected as being specific to ZAP70 were transfected into naïve thymocytes using HVJ-E vectors. Western blot analysis was performed at 48hrs after the transfection. Sequence of siRNA (1): GAAGCUGAAGGCGGAUGGACUAAUU (2): CACGCUUAGCGUCAUCGACAGACAA (3): GCCCUGGGUGCUGACGACAGCUAUU

Figure 5: Delivery of molecules (siRNAs) into cells using HVJ-E vectors.