Figure 6: Uptake and permeability of Curc-NL and TAT-Curc-NL by hCMEC/D3 cell monolayers by radiochemical technique. 6×104 cells were incubated with NL labelled with [3H]-Sm for 3 h at 37°C, 5% CO2. Panel A. Uptake of NL. After incubation, the uptake of Curc-NL or TATCurc- NL by hCMEC/D3 cells has been assessed by following both tracers used, [3H]-Sm (grey bars) by radioactivity counting, and Curc-PEG-lipid (black bars) by mass spectrometry. Panel B. Transcytosis of NL through hCMEC/D3 cell monolayers. The permeability (PE) of Curc-NL and TATCurc- NL across the cell monolayer was calculated for both tracers used, [3H]-Sm (grey bars) and Curc-PEG-lipid (black bars). Each value is the mean of at least three independent experiments and the SDs of means are presented as bars. *=p<0.05. Curc=curcumin-derivative; NL=nanoliposomes; Sm=sphingomyelin.