Figure 6: PMA and cytochalasin D induced Ca+2 secretion from T-leukemia cells is Ca-ATPase dependant. Jurkat cells were treated with the specified chemicals in presence of 45Ca-apatite. Samples were collected at indicated time points after 3 times washing with ice cold PBS, followed by 5 min incubation with 10 mM EDTA in PBS and two times rinsing with ice cold PBS. Samples were immediately processed for radioactivity measurement. Error bar, SEM (n=4); La, Lanthanum chloride; Th, thapsigargin.