Figure 7: Uptake of 45Ca-apatite particles in presence or absence of PMA and Cyt D was clathrin-dependent. Jurkat cells were treated with the specified chemicals in presence of 45Ca-apatite. Samples were collected at indicated time points after 3 times washing with ice cold PBS, followed by 5 min incubation with 10 mM EDTA in PBS and two times rinsing with ice cold PBS. Samples were immediately processed for radioactivity measurement. Error bar, SEM (n=4); Am, Amantadine; PAO, phenylarsine oxide. Uptake of 45Ca-apatite particles in presence or absence of PMA and Cyt D was clathrin-dependent. Jurkat cells were treated with the specified chemicals in presence of 45Ca-apatite. Samples were collected at indicated time points after 3 times washing with ice cold PBS, followed by 5 min incubation with 10 mM EDTA in PBS and two times rinsing with ice cold PBS. Samples were immediately processed for radioactivity measurement. Error bar, SEM (n=4); Am, Amantadine; PAO, phenylarsine oxide.