Figure 4: Macrophages facilitate transport of nanozyme across the BBB in murine model of PD. 3H-catalase nanozyme loaded into BMM (black diamonds) or cell-free nanoparticles (white squares) were injected i.t.v. in MPTP-intoxicated mice. In parallel experiment, BMM loaded with the same amount of nanozyme were injected to healthy animals (crosses). Blood and brain samples at various time points were taken, and radioactivity levels were recorded. Results from N=6 mice per group (± SEM) demonstrating significant increase in cell-incorporated nanozyme transport across the BBB compared to cell-free nanoparticles. The unidirectional influx rate (Ki) for BMM-incorporated and free nanozyme were 0.026μl/g x min and 0.014μl/g x min, respectively. Statistical significance compared to free nanozyme is shown by asterisk (p<0.05).