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Gram staining
Indole test
Methyl Red (MR) test Voges-Proskauer (VP) test Citrate test
Triple Sugar Iron Agar
(TSI) test
Mannitol Motility (MM) test Urea Hydrolysis test Nitrate Reduction test Oxidase test
Sugar Fermentation test
(a) Lactose
(b) Adonitol
(c) Dextrose
(d) Trehalose
(e) Melibiose
(f) Raffinose
(g) Arabinose
(h) Sucrose
(i) Cellobiose
Catalase
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Gram – ve rods
-ve
-ve
-ve
-ve Acid slant/ Acid Butt
- / +
-ve
-ve
+ve
+ve
+ve
+ve
+ve
+ve
+ve
-ve
-ve
+ve
+ve
+ve
+ve
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