Figure 5: PCR amplification using gyrase gene-specific primer sets and genomic DNA from bacterial isolates belonging to the Bacillus cereus/B. thuringiensis group. (a) Lane 1, DNA molecular marker (100 bp DNA ladder, Fermentas); 2 to 8, isolates SGC, SG15, SG20, SG21, P2_1, positive and negative control, respectively, with primer set specific for Bacillus thuringiensis, 9 to 15, isolates SGC, SG15, SG20, SG21, P2_1, positive and negative control, respectively, with primer set specific for Bacillus cereus. Positive controls used for PCR were Bacillus cereus LFB-FIOCRUZ 406T and Bacillus thuringiensis serovar israelensis LFB-FIOCRUZ 584, provided by Fiocruz.