Figure 4: Phylogenetic tree based on the alignment of amino acid sequences (about 97 aa) of 6-hydroxyl-group to 6-oxocyclohex-1-ene-1-carbonyl-CoA. The tree was constructed by using the neighbor-joining (NJ) method in MEGA5 program with substitution model PAM Matrix (Dayhoff). Bootstrap values (1000 replicate runs, shown as %) >70% are listed. The scale bar denotes divergence percentage between sequences. Symbols: clones from GMR75 (●); clones from PTS01 (■). Access numbers of genes from GenBank database reference strains are in parentheses.