Primer Gene target Sequence (5´→ 3´) Direction Expected Amplicon size (bp) Reference
alkF alk GCICAIGARITIRKICAYAA forward 524 [19]
alkR alk GCITGITGITCISWRTGICGYTG reverse
ARHDf α-ARHD TTYRYITGYAIITAYCAYGGITGGG forward 329 [35]
ARHDr α-ARHD AAITKYTCIGCIGSIRMYTTCCA reverse
SP9f bamA CAGTACAAYTTCTACACVACBG forward 300 [32]
ASP1r bamA CMATGCCGATYTCCTGRC reverse
Table 1: Oligonucleotide primers used for PCR assays.