Primer set Initial denaturation Cycle number Denaturation Annealing Extension (72°C) Final extension (72°C) alkF/alkR 97°C, 3 min 35 94°C, 1 min 48°C, 1 min 1 min 5 min ARHDf/ARHDr 97°C, 3 min 35 94°C, 1 min 58°C, 1 min 1 min 5 min SP9f/ASP1r 94°C, 10 min 35 94°C, 30 s 59°C, 45 s 1 min 10 min

Table 2: Polymerase chain reaction programs used in this study.