Parameters Control BS-A BS-J BS-K BS-P
pH 7.0±0.0 8.7±0.0 8.7±0.0 8.9±0.1 8.9±0.0
Biomass yield
(c. f. u. /ml)
12±1x102 94±4x108 89±6x108 90±5x107 68±4x109
Surface tension (mN/m) 61±5.2 27±0.0 27±0.0 28±0.0 27±0.0
Emulsification index (%) - 47±0.8 52±0.8 34±0.8 58±1.4
Biosurfactant yield (g/l) 0.00±0.00 0.79±0.01 (18.2%)* 0.79±0.00 (26.9%)* 0.90±0.01 (22.3%)* 1.86±0.01 (23.5%)*
% reduction in COD 0.0±0.00 82.4±0.90 80.5±0.41 76.0±1.31 80.5±0.78
Total sugars reduction (%) 0.0±0.00 86.2±0.91 82.6±1.10 79.2±0.87 81.4±0.37
Total nitrogen reduction (%) 0.00±0.00 71.3±0.88 62.3±0.87 58.4±0.24 62.8±0.66
Total phosphate reduction (%)     reduction (%)  reduction (%) 0.0±0.00 59.5±0.37 57.0±0.49 48.0±0.24 56.5±0.37
Mean + Standard deviation; n=4
*values presented in the parentheses indicates the percent rise in the yield of biosurfactant production by the respective isolates by replacing 1:3 diluted distillery waste with combination of distillery waste, curd whey and sugar industry effluent in 1:1:1 ratio
Table 3: Biosurfactant production by different isolates in distillery waste combined with curd whey and sugar industry effluent in 1:1:1 ratio (After 120 h of incubation).