Disc No.

Compound

Antibacterial activity (Diameter of inhibition zone in mm)

Antifungal activity (Diameter of inhibition zone in mm)

BS

BT

PSE

 

ESH

AGRO

ACI

RHI

MUC

TILL

1

Fresh crude leaf extract

-

-

-

-

-

-

-

-

-

2

Concentrated crude leaf extract

-

-

-

-

-

-

-

-

-

3

Dried  crude leaf extract dissolved in DMSO

7±0.2

7±0.3

-

-

-

-

-

-

-

4

Concentrated supernatant of fresh crude leaf extract

8±0.4

7±0.2

-

-

-

-

-

-

-

5

Dried supernatant of fresh crude leaf extract dissolved in DMSO

10±0.1

9±0.4

-

-

-

-

-

-

-

6

Standard drug

20±0.7

18±0.8

16±0.5

18±0.6

23±0.4

16±0.2

24±0.3

27±0.4

17±0.2

7

DMSO

-

-

-

-

-

-

-

-

-

8

Fresh crude seed extract

11±0.6

13±0.5

-

-

12±0.4

-

-

10±0.3

-

9

Concentrated crude seed extract

12±0.5

14±0.6

-

-

13±0.2

-

-

14±0.2

11±0.4

10

Seed oil

-

-

-

-

-

-

-

-

-
BS = Bacillus subtilis, BT = Bacillus thuringiensis, PSE = Pseudomonas fluorescens, ESH = Escherichia coli, AGRO = Agrobacterium tumefaciens, ACI = Acinetobactor junii, RHI = Rhizopus oryzae, MUC = Mucor indicus and TILL = Tilletia indica. The experiments were conducted in triplicates and the data were calculated as mean ± SD.
Interpretations of the results: Among all extracts tested, seed extract showed significant and leaf extract showed intermediate activity against both gram-positive bacteria. Whereas, no activity was observed against all test extracts in gram-negative bacteria except in case of A. tumefaciens some activity was obtained against seed extract of H. salicifolia. In case of fungal cultures, among all test extracts only seed extract showed significant activity against Mucor and Tilletia fungus, while in case of Rhizopus no activity was observed against all test extracts.
Table 1: Antibacterial and antifungal activity in leaf, seed extract and seed oil of Seabuckthorn (Hippophae salicifolia D. Don).